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Screening Discovery Platform

Introduction of group leader

David Shum, MS
Master Degree: New York University, USA (2002)
Senior Research Technician, Memorial Sloan Kettering Cancer Center, USA (2002-2004)
Assay Dev. Specialist, Memorial Sloan Kettering Cancer Center, USA (2004-2015)
Head, Assay Development & Screening, Institut Pasteur Korea (2015-present)

Office phone: +82-31-8018-8220
Email: david.shum at ip-korea.org
Office location: R5.29-1

Research Interests

The Screening Discovery Platform (SDP) group provides expertise in the early stages of the drug discovery pipeline for assay development and high-throughput screening. Our research activities enable discovery of small molecules and target identification for drug development, biomarkers, and elucidation of disease mechanisms. SDP works in close collaboration with each investigator, within Institut Pasteur Korea (IPK) and outside collaborators, providing guidance through all stages of the chemical and RNAi screening process:
 
• Assay development
• Assay validation
• Assay automation
• High-content or high-throughput screening
• Data analysis
 
SDP optimizes and validates all screening assays for IPK discovery biology programs such as bacterial (streptococcus pneumoniae, mycobacterium tuberculosis), parasitic (visceral leishmaniasis), and viral research (hepatitis B, EBOLA, MERS-CoV). In 2016, SDP was recognized by Drugs for Neglected Disease initiative (DNDi-IPK) for our role in a multi-collaborative effort to identify small molecules for therapies against visceral leishmaniasis and Chagas disease.
 
Our capabilities/assets include:

Pathogen and biological research conducted in fully-automated robotic platforms located in BSL-2+ and BSL-3 laboratories
Validating and formatting assays to 96/384/1536 well format to perform screening activities
Conducting screening assays covering a wide range of detection readouts including fluorescence, bioluminescence, absorbance, and imaging (confocal and epi-fluorescent)
Small chemical library collection of compounds identified as potential starting points for the development of novel therapeutics. Library is comprised of ~400,000 molecules covering diverse sources including synthetic, natural products, extracts, and FDA approved drugs to early drug discovery programs
Genomic platforms (siRNA and shRNA technology) to identify new targets and signaling pathways as well as uncover mechanism of actions
 

Recent Publications

Identification of Antipneumococcal Molecules Effective Against Different Streptococcus pneumoniae Serotypes Using a Resazurin-Based High-Throughput Screen.
Kim HJ, Kim N, Shum D, Huddar S, Park CM, Jang S
Assay Drug Dev Technol. 2017 Jul;15(5):198-209

Evaluation of Compound Optical Interference in High-Content Screening.
Ibanez G, Calder PA, Radu C, Bhinder B, Shum D, Antczak C, Djaballah H
SLAS Discov. 2018 Apr;23(4):321-329

High-Content Screening of Raw Actinomycete Extracts for the Identification of Antituberculosis Activities.
Heo J, Nam J, Jang J, Shum D, Radu C, Cheng J, Lee H, Suh JW, Delorme V
SLAS Discov. 2017 Feb;22(2):144-154

Development of Cell-Defined Lentivirus-Based Microarray for Mammalian Cells.
Kim HC, Shum D, Seol HS, Jang SJ, Cho SG, Kwon YJ
SLAS Discov. 2017 Jan;22(1):108-113

The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells.
Kim HC, Kim GH, Shum D, Cho SG, Lee EJ, Kwon YJ
Data Brief. 2016 Mar 12;7:673-8