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Introduction of group leader
Office phone: +82-31-8018-8260
Office location: R3.13
The maintenance of IPK’s phenotypic screening facility requires a steady and consistent improvement of advanced cutting-edge screening technologies. The aim of the Technology Development Platform (TDP) group is to further expand high content screening technologies used to investigate the relationship between cell and pathogen. Taking advantage of TDP’s multidisciplinary team composed of biologist, biophysicist, and microscopy specialists, we are developing imaging technologies to study infection, from nano- to macro-scale, in cell and animal models.
Examples of our activities include:
• Using novel imaging resonance energy transfer (RET) techniques, we are investigating the protein-protein interaction (PPI) occurring between pathogen and host protein in living cells, specifically focusing on the multiple pathways leading viruses to subvert the innate immunity of their hosts.
• Developing and adapting physiologically relevant cellular models to 384-well plates for high-content screening, using neurons, astrocytes and microglia derived induced pluripotent stem cells (iPSCs). Using this platform, we are studying the role of brain cell populations in the initiation of innate immune responses to neurotropic viruses.
• Taking advantage of near infrared fluorescent protein for their low absorption coefficient and auto-fluorescence to generate novel animal models reporting inflammatory responses in their brain. Furthermore, we are exploring alternative multimodal strategy for in vivo and in vitro imaging, further closing the scale gap between live small animal imaging and post mortem histology
Glioblastoma-secreted soluble CD44 activates tau pathology in the brain.
Lim S, Kim D, Ju S, Shin S, Cho IJ, Park SH, Grailhe R, Lee C, Kim YK
Exp Mol Med. 2018 Apr 6;50(4):5
Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells.
Lee H, Radu C, Han JW, Grailhe R
J Vis Exp. 2017 Oct 4;(128)
Regulation of NF-κB by the p105-ABIN2-TPL2 complex and RelAp43 during rabies virus infection.
Besson B, Sonthonnax F, Duchateau M, Ben Khalifa Y, Larrous F, Eun H, Hourdel V, Matondo M, Chamot-Rooke J, Grailhe R, Bourhy H
PLoS Pathog. 2017 Oct 30;13(10):e1006697
Nanoluciferase signal brightness using furimazine substrates opens bioluminescence resonance energy transfer to widefield microscopy.
Kim J, Grailhe R
Cytometry A. 2016 Aug;89(8):742-6
Dimerization, oligomerization, and aggregation of human amyotrophic lateral sclerosis copper/zinc superoxide dismutase 1 protein mutant forms in live cells.
Kim J, Lee H, Lee JH, Kwon DY, Genovesio A, Fenistein D, Ogier A, Brondani V, Grailhe R
J Biol Chem. 2014 May 23;289(21):15094-103