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Assays & Technologies

Cancer Biology Research Laboratory

Hepatocellular carcinoma (HCC) stem cell assay [Code No: IPK0601]

This assay can be used for identifying compounds specifically targeting HCC and liver cancer stem cells (LCSC) versus normal hepatocytes in a HCC-mixed population. This approach is a valid in vitro model for monitoring heterocellular interactions. To distinguish each population in a heterogeneous HCC-population, CHALV1 and AFP, which are HCC markers, and CD133 as an appropriate LCSC marker, have been used in the HCC assay.

3D culture system in HTS/HCS [Code No: IPK0602]

In this assay, 3D spheroids are utilized for drug screening of HCC. Three dimensional cultures of hepatocytes are the proper culture models to sustain liver-specific functions, as well as liver-specific architecture, similar to those found in vivo. Features of spheroids are extracted at 3 different levels: the signal level, the cell level and the organoids level.

Clonogenic survival assay [Code No: IPK0603]

A clonogenic assay is a cell biology technique for studying the effectiveness of specific agents on the survival and proliferation of cells. It is frequently used in cancer research to determine the effect of drugs or radiation on proliferating tumor cells.

Tumor xenograft assay in nude mouse [Code No: IPK0604]

This assay is routinely used for detection of drug efficacy in vivo. In this assay, single-cell suspensions are injected into hind legs of 5-week old nude mice. When tumors reach a volume of 150-250mm2, mice are randomly allocated as experiment design.

Caspase 3 activity assay [Code No: IPK0605]

This assay is used to detect apoptosis. Drug-treated lysates are incubated with Ac-DEVD-pNA for 4 hours, and then caspase 3 activity is measured at 405nm.

In vitro migration assay [Code No: IPK0606]

This assay is used to detect migration ability in various cancer cells. Because migration ability can induce serious results in cancer patients, it is important to evaluate it in drug discovery.

DNA damage assay & micro nuclei assay [Code No: IPK0607]

This assay is used to detect DNA damage and genotoxicity after exposure to drugs. To detect DNA damage, p-H2AX are stained in drug-exposed cells. Micro nuclei are detected by Hoechst staining.

Cell cycle analysis assay [Code No: IPK0608]

Most drugs induce changes in cell cycle distribution. To evaluate these changes, suspended cells stained with PI (propidium iodide) are analyzed by FACS.

Cell cycle analysis [Code No: IPK0611]

Most drugs induce changes in cell cycle distribution. For this end, suspended cell which were stained with PI (Propidium iodide) were analyzed FACS.

EdU assay [Code No: IPK0612]

This assay uses EdU staining to detect proliferation activity after exposure to drugs. The results are analyzed on an Operetta system.

ROS detection assay [Code No: IPK0613]

Reactive oxygen species (ROS) is major inducer of apoptosis. In this assay, ROS are detected by HCS or FACS after staining of DCF.

Autophagy detection [Code No: IPK0614]

Balance of autophagy is important for cell homeostasis. Studies have shown that accumulation of autophagosome induces cell death. For detection of autophagy, active form-LC3B is measured by HCS or Western blot.