알림·소식
알림·소식
"Live! A new system for studying Hepatitis C virus in vitro"
2010-05-11
- Title: "Live! A new system for studying Hepatitis C virus in vitro"
- Speaker: Dr Christopher Jones, Rockefeller University, USA(* CV attached )
- Date & Time: Tuesday, May 11, 2010/ 4:00pm~5:00pm
- Venue: Auditorium at Institut Pasteur Korea, Pangyo Techno-valley
- Inquiries: Sangsook Oh(오상숙), Scientific Affairs, Institut Pasteur Korea
(Tel: 031-8018-8042).
*If you’d like to join the seminar, please contact at sangsook@ip-korea.org
- Abstract: Much progress has been made in recent years in developing relatively robust cell culture systems for the production of infectious hepatitis C virus (HCV). Traditional techniques for visualizing HCV infection in cell culture generally involve terminal processing, such as cell lysis or fixation, and thus the destruction of potentially precious samples. Live cell imaging avoids this, but requires the use of an HCV reporter genome, which necessitates genetic modification and may impact infectivity or replication kinetics. As an alternative approach, we have developed a reporter cell system that allows visualization of infection in live or fixed cells using virtually any HCVcc genome. The system depends on the NS3-4A-mediated cleavage and subsequent relocalization of a mitochondrially-tethered fluorescent protein substrate. In the presence of an NS5B inhibitor to prevent replication, translation of incoming HCV RNA can be readily visualized within 12 hours post-infection, demonstrating the sensitivity of this system. We explore the use of the reporter cell technology for a number of applications, including live cell imaging of virus infection and clearance, real time visualization of cell-to-cell spread, and de novo infection of primary hepatocyte cell cultures.
- Speaker: Dr Christopher Jones, Rockefeller University, USA(* CV attached )
- Date & Time: Tuesday, May 11, 2010/ 4:00pm~5:00pm
- Venue: Auditorium at Institut Pasteur Korea, Pangyo Techno-valley
- Inquiries: Sangsook Oh(오상숙), Scientific Affairs, Institut Pasteur Korea
(Tel: 031-8018-8042).
*If you’d like to join the seminar, please contact at sangsook@ip-korea.org
- Abstract: Much progress has been made in recent years in developing relatively robust cell culture systems for the production of infectious hepatitis C virus (HCV). Traditional techniques for visualizing HCV infection in cell culture generally involve terminal processing, such as cell lysis or fixation, and thus the destruction of potentially precious samples. Live cell imaging avoids this, but requires the use of an HCV reporter genome, which necessitates genetic modification and may impact infectivity or replication kinetics. As an alternative approach, we have developed a reporter cell system that allows visualization of infection in live or fixed cells using virtually any HCVcc genome. The system depends on the NS3-4A-mediated cleavage and subsequent relocalization of a mitochondrially-tethered fluorescent protein substrate. In the presence of an NS5B inhibitor to prevent replication, translation of incoming HCV RNA can be readily visualized within 12 hours post-infection, demonstrating the sensitivity of this system. We explore the use of the reporter cell technology for a number of applications, including live cell imaging of virus infection and clearance, real time visualization of cell-to-cell spread, and de novo infection of primary hepatocyte cell cultures.