Research & Technology
Research & Technology
Research & Technology
at a Glance
- Discovery Biology
- Translational Research
- Research Infra Center
- Technology & Infrastructure
Research & Technology
The Advanced Biomedical Research Laboratory (ABRL) is committed to serving as a platform to develop breakthrough discoveries. ABRL’s area of expertise is liver cancer. To combat the limitations of existing liver cancer therapies, ABRL took a holistic view of tumors, constructing a 3-dimensional (3D) tumor microenvironment in vitro to fully understand tumor development, progression, chemo-resistance and treatment response which can be used as a platform for anti-cancer drug discovery, improving personalized cancer therapy.
In general, liver cancer is accompanied by fibrosis, cirrhosis and liver-related disease. Yet, scientists have mainly focused on elimination of hepatocellular cacinoma (HCC) for liver cancer therapy. ABRL challenged various strategies such as anti-fibrotic, anti-inflammatory, molecular target approaches and regulation of metabolic dysregulation in liver disease using small molecule, recombinant proteins, natural compounds, and traditional oriental medicines for treatment of HCC and prevention of HCC development in progressive fibrotic liver diseases.
ABRL also worked to establish 3D spheroid models for development of novel therapeutic drugs for infectious diseases caused by viruses, parasites, bacteria, etc., since 3D tissue model serves as an effective tool for studying infectious diseases.
Our present activity focuses on:
• Investigating reciprocal crosstalk between the tumor and tumor microenvironment to reveal new information on infrastructure that supports tumor existence
• Discovering new biomarker(s) for early diagnosis of liver cancer and novel target(s) for liver cancer therapy
• Establishing 3D tumor microenvironment platform for anti-liver cancer drug discovery
• Development of innovative strategy for reprogramming of fibrotic microenvironment
• Applying 3D tissue models to infectious disease research
Haengran Seo, Ph.D.
Sanghwa Kim, PhD
Suyeon Lee, PhD
Yeonhwa Song, PhD
Taemoo Heo, MS
Expression of HYOU1 via Reciprocal Crosstalk between NSCLC Cells and HUVECs Control Cancer Progression and Chemoresistance in Tumor Spheroids
Mol Cells (2021) 44(1):50-62 PMID: 33455947, PMCID: PMC7854178
Minji Lee, Yeonhwa Song, Inhee Choi, Su-Yeon Lee, Sanghwa Kim, Se-Hyuk Kim, Jiho Kim, Haeng Ran Seo
Argininosuccinate synthase 1 suppresses tumor progression through activation of PERK/eIF2α/ATF4/CHOP axis in hepatocellular carcinoma
J Exp Clin Cancer Res. (2021) 40(1):127 PMCID: PMC8035787
Sanghwa Kim, Minji Lee, Yeonhwa Song, Su-Yeon Lee, Inhee Choi, I-Seul Park, Jiho Kim, Jin-Sun Kim, Kang Mo Kim*, Haeng Ran Seo*.
Identification of hepatic fibrosis inhibitors through morphometry analysis of a hepatic multicellular spheroids model
Sci Rep. (2021) 11(1):10931 PMCID: PMC8149639
Yeonhwa Song#, Sanghwa Kim#, Jinyeong Heo, David Shum, Su-Yeon Lee, Minji Lee, A-Ram Kim, Haeng Ran Seo (# These authors have contributed equally).
Anti-Cancer Effect of Moroccan Cobra Naja haje Venom and Its Fractions against Hepatocellular Carcinoma in 3D Cell Culture.
Toxins (Basel). (2021) 13(6):402 PMID: 34199838, PMCID: PMC8229680
Ayoub Lafnoune, Su-Yeon Lee, Jin-Yeong Heo, Imane Gourja, Bouchra Darkaoui, Zaineb Abdelkafi-Koubaa, Fatima Chgoury, Khadija Daoudi, Salma Chakir, Rachida Cadi, Khadija Mounaji, Najet Srairi-Abid, Naziha Marrakchi, David Shum, Haeng-Ran Seo, Naoual Oukkache.
Inhibitors of Na +/K + ATPase Exhibit Antitumor Effects on Multicellular Tumor Spheroids of Hepatocellular Carcinoma
Sci Rep. (2020) 10(1):5318 PMCID: PMC7093469
Yeonhwa Song, Su-Yeon Lee, Sanghwa Kim, Inhee Choi, Se-Hyuk Kim, David Shum, Jinyeong Heo, A-Ram Kim, Kang Mo Kim, and Haeng Ran Seo.
Chromenopyrimidinone Controls Stemness and Malignancy by Suppressing CD133 Expression in Hepatocellular Carcinoma
Cancers. (2020) 12(5):1193 PMCID: PMC7281429
Yeonhwa Song, Sanghwa Kim, Hyeryon Lee, Joo Hwan No, Hyung Chul Ryu, Jason Kim, Jee Woong Lim, Moonhee Kim, Inhee Choi*, Haeng Ran Seo*.
Identification of inhibitors of Bcl-2 family protein-protein interaction by combining the BRET screening platform with virtual screening
Biochemical and Biophysical Research Communications (2020) 527(3):709-715 PMID: 32423828
I-Seul Park, Haeng Ran Seo, Kideok Kim, Honggun Lee, David Shum, Inhee Choi*, Jiho Kim*.
GSK-3 beta regulates the endothelial-to-mesenchymal transition via reciprocal crosstalk between NSCLC cells and HUVECs in multicellular tumor spheroid models
J. Exp. Clin. Cancer Res. (2019) 38(1):46 PMCID: PMC6359813
Se-Hyuk Kim, Yeonhwa Song, Haeng Ran Seo.
Actinomycin D Inhibits the Expression of the cystine/glutamate Transporter xCT via Attenuation of CD133 Synthesis in CD133 + HCC
Chem Biol Interact. (2019) 309:108713 PMID: 31226288
Yeonhwa Song, I-Seul Park, Jiho Kim, Haeng Ran Seo
Recent advances in aerosol gene delivery systems using non-viral vectors for lung cancer therapy
Expert Opinion on Drug Delivery (2019) 16(7): 757-772 PMID: 31282221
Ah Young Lee, Myung-Haing Cho, Sanghwa Kim
Patient-derived multicellular tumor spheroids towards optimized treatment for patients with hepatocellular carcinoma
J Exp Clin Cancer Res (2018) 37(1):109 PMID: 29801504, PMCID: PMC5970513
Yeonhwa Song, Jin-Sun Kim, Se-Hyuk Kim, Yoon Kyung Park, Eunsil Yu, Ki-Hun Kim, Eul-Ju Seo, Heung-Bum Oh, Han Chu Lee, Kang Mo Kim, Haeng Ran Seo
Oxytetracycline Have the Therapeutic Efficiency in CD133 + HCC Population Through Suppression CD133 Expression by Decreasing of Protein Stability of CD133
Sci Rep. (2018) 8(1) PMID: 30382122, PMCID: PMC6208387
Yeonhwa Song, In-Ki Kim, Inhee Choi, Se-Hyuk Kim, Haeng Ran Seo
Identification of radiation-induced EndMT inhibitors through cell-based phenomic screening
FEBS Open Bio (2018) 9(1): 82-91 PMID: 30652076, PMCID: PMC6325571
Yeonhwa Song, Su-Yeon Lee, A-Ram Kim, Sanghwa Kim, Jinyeong Heo, David Shum, Se-Hyuk Kim, Inhee Choi, Yoon-Jin Lee, Haeng Ran Seo
CD133 confers cancer stem-like cell properties by stabilizing EGFR-AKT signaling in hepatocellular carcinoma
Cancer Lett. (2017) 389:1-10 PMID: 28034805
Jang JW, Song Y, Kim SH, Kim JS, Kim KM, Choi EK, Kim J, Seo HR
Sulfasalazine attenuates evading anticancer response of CD133-positive hepatocellular carcinoma cells.
J Exp Clin Cancer Res (2017) 36(1):38 PMID: 28253902, PMCID: PMC5335796
Song Y, Jang J, Shin TH, Bae SM, Kim JS, Kim KM, Myung SJ, Choi EK, Seo HR.
TGF-β-independent CTGF induction regulates cell adhesion mediated drug resistance by increasing collagen I in HCC.
Oncotarget. (2017) 21650-21662 PMID: 28423507, PMCID: PMC5400613
Song Y, Kim JS, Choi EK, Kim J, Kim KM, Seo HR
Hepatocellular carcinoma-targeted drug discovery through image-based phenotypic screening in co-cultures of HCC cells with hepatocytes
BMC Cancer (2016) 16(1):810 PMID: 27756242, PMCID: PMC5069815
Jang JW, Song Y, Kim KM, Kim JS, Choi EK, Kim J, Seo H
Activated hepatic stellate cells play pivotal roles in hepatocellular carcinoma cell chemoresistance and migration in multicellular tumor spheroids.
Scientific reports (2016) 6:36750. PMID: 27853186, PMCID: 5113076
Song YH, Kim SH, Kim KM, Choi EK, Kim J, Seo HR
A pathway-based approach for identifying biomarkers of tumor progression to trastuzumab-resistant breast cancer.
Cancer Letters. (2015) 356 (2 Pt B):880-90 PMID: 25449779
Nam S, Chang HR, Jung HR, Gim Y, Kim NY, Grailhe R, Seo HR, Park HS, Balch C, Lee J, Park I, Jung SY, Jeong KC, Powis G, Liang H, Lee ES, Ro J, Kim YH.
Mutual regulation between DNA-PKcs and Snail1 leads to increased genomic instability and aggressive tumor characteristics
Cell Death & Disease (2013) 4:e517 PMID: 23449453, PMCID: PMC3734834
Seo HR, Pyun BJ, Lee HJ, Jin YB, Kim EJ, Kim NH, Kim HS, Nam HW, Yook JI, Lee YS
Inhibition of Snail1-DNA-PKcs Protein-Protein Interface Sensitizes Cancer Cells and Inhibits Tumor Metastasis
Journal of Biological Chemistry (2013) 288:32506-32516 PMID: 24085291, PMCID: PMC3820884
Kang GY, Pyun BJ, Seo HR, Jin YB, Lee HJ, Lee YJ, Lee YS
Radiosensitization by celastrol is mediated by modification of antioxidant thiol molecules
Chemico-Biological Interactions (2011) 193:34-42 PMID: 21570383
Seo HR, Seo WD, Pyun BJ, Lee BW, Jin YB, Park KH, Seo EK, Lee YJ, Lee YS
Synthesis and cytotoxicity of 2-phenylquinazolin-4(3H)-one derivatives
European Journal of Medicinal Chemistry (2011) 46:3900-3908 PMID: 21704436
Rhee HK, Yoo JH, Lee E, Kwon YJ, Seo HR, Lee YS, Choo HY
Cyclin G1 overcomes radiation-induced G2 arrest and increases cell death through transcriptional activation of cyclin B1
Cell Death & Differentiation (2006) 13:1475-1484 PMID: 16322753
Seo HR, Lee DH, Lee HJ, Baek M, Bae S, Soh JW, Lee SJ, Kim J, Lee YS
p27Cip/Kip is involved in hsp25 or inducible hsp70 mediated adaptive response by low dose radiation
Journal of Radiation Research (2006) 47:83-90 PMID: 16571921
Seo HR, Chung HY, Lee YJ, Bae S, Lee SJ, Lee YS
) Heat shock protein 25 or inducible heat shock protein 70 activates heat shock factor 1: dephosphorylation on serine 307 through inhibition of ERK1/2 phosphorylation
Journal of Biological Chemistry (2006) 281:17220-17227 PMID: 16624816
Seo HR, Chung DY, Lee YJ, Lee DH, Kim JI, Bae S, Chung HY, Lee SJ, Jeoung D, Lee YS